In order to realize the accurate evaluation of queue posture and action, aiming at the problem that it is difficult to accurately measure the three-dimensional human posture characteristics such as kick height in the training scene, this paper proposed a queue three-dimensional posture feature detection method based on binocular information. The method is divided into two stages: the 2D pose estimation stage and binocular stereo matching stage. In order to improve the accuracy of 2D human pose detection, it designed a 2D pose estimation model based on the improved HRNet network. Firstly, it introduced spatial channel attention in the back-bone network to enhance feature extraction capabilities. The feature fusion layer used an adaptive spatial feature fusion module to fuse multi-scale features of the network. Secondly, it used the unbiased data processing method to encode and decode the heat map to reduce the statistical error of the data. Finally, it adopted a coarse-to-fine multi-stage supervision method during model training to improve the detection accuracy of key points. Based on the 2D pose estimation model, it used the standard correlation matching function to achieve binocular stereo matching, and then obtained the 3D human pose through coordinate transformation. The experimental results show that the improved pose estimation network has better accuracy, the accuracy of the COCO dataset reaches 77.1%, and the detection accuracy of the self-made queue training dataset reaches 86.3%, which are respectively 2.2% and 3.1% higher than the original network. In the kick height experiment of the three-dimensional human body posture, the average relative error measured by the proposed method is 2.5%, which fully verifies the effectiveness of the algorithm.